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pax8 polyclonal antibody  (Proteintech)


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    Proteintech pax8 polyclonal antibody
    Pax8 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pax8 polyclonal antibody/product/Proteintech
    Average 96 stars, based on 515 article reviews
    pax8 polyclonal antibody - by Bioz Stars, 2026-02
    96/100 stars

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    ( a – d , top row) Diagrams depicting the development of the three regions (extraovarian rete [ER], magenta; connecting rete [CR], cyan; intraovarian rete [IOR], yellow) of the RO from E16.5 to P7 in the whole ovarian complex (ovary, light pink; oviduct, gray). ( a –d, bottom rows) Maximum intensity projection from confocal Z-stacks of whole ovary/mesonephros complexes at E16.5 ( a ), E18.5 ( b ), P0 ( c ), and P7 ( d ) immunostained for <t>PAX8</t> (yellow), GFRa1 (cyan), and KRT8 (magenta). ( a – d , second row) are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the CR and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.
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    Figure 1. The rete ovarii (RO) undergoes dynamic changes during development alongside the ovary. (a–d, top row) Diagrams depicting the development of the three regions (extraovarian rete [ER], magenta; connecting rete [CR], cyan; intraovarian rete [IOR], yellow) of the RO from E16.5 to P7 in the whole ovarian complex (ovary, light pink; oviduct, gray). (a–d, bottom rows) Maximum intensity projection from confocal Z-stacks of whole ovary/ mesonephros complexes at E16.5 (a), E18.5 (b), P0 (c), and P7 (d) immunostained for <t>PAX8</t> (yellow), GFRa1 (cyan), and KRT8 (magenta). (a–d, second row) are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the CR and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.
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    Figure 1. The rete ovarii (RO) undergoes dynamic changes during development alongside the ovary. (a–d, top row) Diagrams depicting the development of the three regions (extraovarian rete [ER], magenta; connecting rete [CR], cyan; intraovarian rete [IOR], yellow) of the RO from E16.5 to P7 in the whole ovarian complex (ovary, light pink; oviduct, gray). (a–d, bottom rows) Maximum intensity projection from confocal Z-stacks of whole ovary/ mesonephros complexes at E16.5 (a), E18.5 (b), P0 (c), and P7 (d) immunostained for <t>PAX8</t> (yellow), GFRa1 (cyan), and KRT8 (magenta). (a–d, second row) are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the CR and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.
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    anti pax8 rabbit polyclonal antibody  (Proteintech)
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    Figure 1. The rete ovarii (RO) undergoes dynamic changes during development alongside the ovary. (a–d, top row) Diagrams depicting the development of the three regions (extraovarian rete [ER], magenta; connecting rete [CR], cyan; intraovarian rete [IOR], yellow) of the RO from E16.5 to P7 in the whole ovarian complex (ovary, light pink; oviduct, gray). (a–d, bottom rows) Maximum intensity projection from confocal Z-stacks of whole ovary/ mesonephros complexes at E16.5 (a), E18.5 (b), P0 (c), and P7 (d) immunostained for <t>PAX8</t> (yellow), GFRa1 (cyan), and KRT8 (magenta). (a–d, second row) are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the CR and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.
    Anti Pax8 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti pax8 rabbit polyclonal antibody - by Bioz Stars, 2026-02
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    anti pax8 rabbit polyclonal  (Proteintech)
    96
    Proteintech anti pax8 rabbit polyclonal
    Figure 1. The rete ovarii (RO) undergoes dynamic changes during development alongside the ovary. (a–d, top row) Diagrams depicting the development of the three regions (extraovarian rete [ER], magenta; connecting rete [CR], cyan; intraovarian rete [IOR], yellow) of the RO from E16.5 to P7 in the whole ovarian complex (ovary, light pink; oviduct, gray). (a–d, bottom rows) Maximum intensity projection from confocal Z-stacks of whole ovary/ mesonephros complexes at E16.5 (a), E18.5 (b), P0 (c), and P7 (d) immunostained for <t>PAX8</t> (yellow), GFRa1 (cyan), and KRT8 (magenta). (a–d, second row) are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the CR and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.
    Anti Pax8 Rabbit Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pax8 rabbit polyclonal/product/Proteintech
    Average 96 stars, based on 1 article reviews
    anti pax8 rabbit polyclonal - by Bioz Stars, 2026-02
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    Image Search Results


    ( a – d , top row) Diagrams depicting the development of the three regions (extraovarian rete [ER], magenta; connecting rete [CR], cyan; intraovarian rete [IOR], yellow) of the RO from E16.5 to P7 in the whole ovarian complex (ovary, light pink; oviduct, gray). ( a –d, bottom rows) Maximum intensity projection from confocal Z-stacks of whole ovary/mesonephros complexes at E16.5 ( a ), E18.5 ( b ), P0 ( c ), and P7 ( d ) immunostained for PAX8 (yellow), GFRa1 (cyan), and KRT8 (magenta). ( a – d , second row) are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the CR and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.

    Journal: eLife

    Article Title: Rediscovering the rete ovarii , a secreting auxiliary structure to the ovary

    doi: 10.7554/eLife.96662

    Figure Lengend Snippet: ( a – d , top row) Diagrams depicting the development of the three regions (extraovarian rete [ER], magenta; connecting rete [CR], cyan; intraovarian rete [IOR], yellow) of the RO from E16.5 to P7 in the whole ovarian complex (ovary, light pink; oviduct, gray). ( a –d, bottom rows) Maximum intensity projection from confocal Z-stacks of whole ovary/mesonephros complexes at E16.5 ( a ), E18.5 ( b ), P0 ( c ), and P7 ( d ) immunostained for PAX8 (yellow), GFRa1 (cyan), and KRT8 (magenta). ( a – d , second row) are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the CR and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.

    Article Snippet: Antibody , PAX8 (rabbit polyclonal) , Proteintech , A10336-1-AP; RRID: AB_2918972 , (1:500).

    Techniques:

    Maximum intensity projection from confocal Z-stacks of whole ovary/mesonephros complexes at E16.5 ( a ), E18.5 ( b ), P0 ( c ), and P7 ( d ) immunostained for PAX8 (yellow), GFRa1 (cyan), and KRT8 (magenta). Top row are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the connecting rete (CR) and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.

    Journal: eLife

    Article Title: Rediscovering the rete ovarii , a secreting auxiliary structure to the ovary

    doi: 10.7554/eLife.96662

    Figure Lengend Snippet: Maximum intensity projection from confocal Z-stacks of whole ovary/mesonephros complexes at E16.5 ( a ), E18.5 ( b ), P0 ( c ), and P7 ( d ) immunostained for PAX8 (yellow), GFRa1 (cyan), and KRT8 (magenta). Top row are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the connecting rete (CR) and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.

    Article Snippet: Antibody , PAX8 (rabbit polyclonal) , Proteintech , A10336-1-AP; RRID: AB_2918972 , (1:500).

    Techniques:

    ( a ) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for ENDOMUCIN (magenta) and PAX8 (cyan). Bottom panel is an optical section showing vasculature tightly surrounding the distal dilated tip (DDT) of the EOR (magenta). ( b ) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for aSMA (magenta) and KRT8 (cyan). Bottom panel is an optical section showing the EOR tightly ensheathed by smooth muscle (magenta). ( c ) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for TUJ1 (magenta) and PAX8 (cyan). Bottom panel is an optical section showing direct contacts between the EOR and neuronal projections (magenta). ( d ) EOR at P0 immunostained for PAX8 (yellow), F4/80 (magenta), and LYVE-1 (cyan). Top and bottom panels are maximum intensity projection of the confocal Z-stack. Bottom panel shows the absence of LYVE1 macrophages (cyan) proximate to the EOR, in contrast to the closely associated F4/80 macrophages (magenta in top image). Arrowheads show regions devoid of LYVE1 macrophages where F4/80 macrophages are present. Scale bar – 100 um.

    Journal: eLife

    Article Title: Rediscovering the rete ovarii , a secreting auxiliary structure to the ovary

    doi: 10.7554/eLife.96662

    Figure Lengend Snippet: ( a ) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for ENDOMUCIN (magenta) and PAX8 (cyan). Bottom panel is an optical section showing vasculature tightly surrounding the distal dilated tip (DDT) of the EOR (magenta). ( b ) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for aSMA (magenta) and KRT8 (cyan). Bottom panel is an optical section showing the EOR tightly ensheathed by smooth muscle (magenta). ( c ) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for TUJ1 (magenta) and PAX8 (cyan). Bottom panel is an optical section showing direct contacts between the EOR and neuronal projections (magenta). ( d ) EOR at P0 immunostained for PAX8 (yellow), F4/80 (magenta), and LYVE-1 (cyan). Top and bottom panels are maximum intensity projection of the confocal Z-stack. Bottom panel shows the absence of LYVE1 macrophages (cyan) proximate to the EOR, in contrast to the closely associated F4/80 macrophages (magenta in top image). Arrowheads show regions devoid of LYVE1 macrophages where F4/80 macrophages are present. Scale bar – 100 um.

    Article Snippet: Antibody , PAX8 (rabbit polyclonal) , Proteintech , A10336-1-AP; RRID: AB_2918972 , (1:500).

    Techniques:

    ( a–d ) Cells of the CR acquire E-CADHERIN soon after birth. Maximum intensity projection from confocal Z-stacks of whole ovary/mesonephros complexes at E16.5-P7 immunostained for PAX8 (magenta) and E-CADHERIN (cyan). Arrowhead shows the region of the CR where E-CADHERIN expression is negligible at E16.5 ( a ), low at E18.5 ( b ), and fully expressed at P0 ( c ) and P7 ( d ). ( e ) Optical section from confocal Z-stacks of RO complexes at P0 immunostained for E-CADHERIN (yellow), CNN1 (magenta), and aSMA (cyan). Boxed regions show the absence of CNN1 in the aSMA+ sheath around the EOR at P0, and ( f ) its acquisition by P7, suggesting gain of contractility. ( g ) The RO expresses cilia marker ARL13b by P0. Maximum intensity projection from confocal Z-stacks of RO complexes at E18.5 immunostained for E-CADHERIN (cyan) and ARL13b (magenta). Outlined boxes show all regions of the EOR have ciliated cells. Scale bar – 100 um. ( h ) Airyscan optical section from confocal Z-stacks of RO complexes at E18.5 immunostained for KRT8 (cyan) and ARL13b (magenta). Scale bar – 25 um.

    Journal: eLife

    Article Title: Rediscovering the rete ovarii , a secreting auxiliary structure to the ovary

    doi: 10.7554/eLife.96662

    Figure Lengend Snippet: ( a–d ) Cells of the CR acquire E-CADHERIN soon after birth. Maximum intensity projection from confocal Z-stacks of whole ovary/mesonephros complexes at E16.5-P7 immunostained for PAX8 (magenta) and E-CADHERIN (cyan). Arrowhead shows the region of the CR where E-CADHERIN expression is negligible at E16.5 ( a ), low at E18.5 ( b ), and fully expressed at P0 ( c ) and P7 ( d ). ( e ) Optical section from confocal Z-stacks of RO complexes at P0 immunostained for E-CADHERIN (yellow), CNN1 (magenta), and aSMA (cyan). Boxed regions show the absence of CNN1 in the aSMA+ sheath around the EOR at P0, and ( f ) its acquisition by P7, suggesting gain of contractility. ( g ) The RO expresses cilia marker ARL13b by P0. Maximum intensity projection from confocal Z-stacks of RO complexes at E18.5 immunostained for E-CADHERIN (cyan) and ARL13b (magenta). Outlined boxes show all regions of the EOR have ciliated cells. Scale bar – 100 um. ( h ) Airyscan optical section from confocal Z-stacks of RO complexes at E18.5 immunostained for KRT8 (cyan) and ARL13b (magenta). Scale bar – 25 um.

    Article Snippet: Antibody , PAX8 (rabbit polyclonal) , Proteintech , A10336-1-AP; RRID: AB_2918972 , (1:500).

    Techniques: Expressing, Marker

    ( a ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) extraovarian rete (EOR) at E18.5 using hybridization chain reaction (HCR) for the detection of Clu expression (magenta). ( b ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at E18.5 using HCR for Stxbp2 (magenta). ( c ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for Clu (magenta). ( d ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for Stxbp2 (magenta). ( e ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-Cre; Rosa26mTmG (cyan) EOR at E18.5 immunostained for STX3 (magenta). Outlined higher resolution image acquired with Airyscan. ( f ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-Cre; Rosa26mTmG (cyan) EOR at E18.5 immunostained for RAB11 (magenta). Outlined higher resolution image acquired with Airyscan. Scale bar – 100 um.

    Journal: eLife

    Article Title: Rediscovering the rete ovarii , a secreting auxiliary structure to the ovary

    doi: 10.7554/eLife.96662

    Figure Lengend Snippet: ( a ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) extraovarian rete (EOR) at E18.5 using hybridization chain reaction (HCR) for the detection of Clu expression (magenta). ( b ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at E18.5 using HCR for Stxbp2 (magenta). ( c ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for Clu (magenta). ( d ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for Stxbp2 (magenta). ( e ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-Cre; Rosa26mTmG (cyan) EOR at E18.5 immunostained for STX3 (magenta). Outlined higher resolution image acquired with Airyscan. ( f ) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-Cre; Rosa26mTmG (cyan) EOR at E18.5 immunostained for RAB11 (magenta). Outlined higher resolution image acquired with Airyscan. Scale bar – 100 um.

    Article Snippet: Antibody , PAX8 (rabbit polyclonal) , Proteintech , A10336-1-AP; RRID: AB_2918972 , (1:500).

    Techniques: Hybridization, Expressing

    ( a ) Maximum intensity projection of confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) extraovarian rete (EOR) at E18.5 using hybridization chain reaction (HCR) for the detection of IGFBP2 expression (magenta). ( b ) Maximum intensity projection of confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for the detection of IGFBP2 expression (magenta). Bottom panels are outlined. Higher resolution images acquired with Airyscan. Scale bar top panel – 100 um. Scale bar bottom panel – 25 um.

    Journal: eLife

    Article Title: Rediscovering the rete ovarii , a secreting auxiliary structure to the ovary

    doi: 10.7554/eLife.96662

    Figure Lengend Snippet: ( a ) Maximum intensity projection of confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) extraovarian rete (EOR) at E18.5 using hybridization chain reaction (HCR) for the detection of IGFBP2 expression (magenta). ( b ) Maximum intensity projection of confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for the detection of IGFBP2 expression (magenta). Bottom panels are outlined. Higher resolution images acquired with Airyscan. Scale bar top panel – 100 um. Scale bar bottom panel – 25 um.

    Article Snippet: Antibody , PAX8 (rabbit polyclonal) , Proteintech , A10336-1-AP; RRID: AB_2918972 , (1:500).

    Techniques: Hybridization, Expressing

    Journal: eLife

    Article Title: Rediscovering the rete ovarii , a secreting auxiliary structure to the ovary

    doi: 10.7554/eLife.96662

    Figure Lengend Snippet:

    Article Snippet: Antibody , PAX8 (rabbit polyclonal) , Proteintech , A10336-1-AP; RRID: AB_2918972 , (1:500).

    Techniques: Sequencing, Software

    Figure 1. The rete ovarii (RO) undergoes dynamic changes during development alongside the ovary. (a–d, top row) Diagrams depicting the development of the three regions (extraovarian rete [ER], magenta; connecting rete [CR], cyan; intraovarian rete [IOR], yellow) of the RO from E16.5 to P7 in the whole ovarian complex (ovary, light pink; oviduct, gray). (a–d, bottom rows) Maximum intensity projection from confocal Z-stacks of whole ovary/ mesonephros complexes at E16.5 (a), E18.5 (b), P0 (c), and P7 (d) immunostained for PAX8 (yellow), GFRa1 (cyan), and KRT8 (magenta). (a–d, second row) are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the CR and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.

    Journal: eLife

    Article Title: Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary

    doi: 10.7554/elife.96662

    Figure Lengend Snippet: Figure 1. The rete ovarii (RO) undergoes dynamic changes during development alongside the ovary. (a–d, top row) Diagrams depicting the development of the three regions (extraovarian rete [ER], magenta; connecting rete [CR], cyan; intraovarian rete [IOR], yellow) of the RO from E16.5 to P7 in the whole ovarian complex (ovary, light pink; oviduct, gray). (a–d, bottom rows) Maximum intensity projection from confocal Z-stacks of whole ovary/ mesonephros complexes at E16.5 (a), E18.5 (b), P0 (c), and P7 (d) immunostained for PAX8 (yellow), GFRa1 (cyan), and KRT8 (magenta). (a–d, second row) are composite images, while the third row shows separate panels for KRT8 and the bottom row shows panels for GFRa1. *Note that GFRa1 and KRT8 do not co-localize and are specific to the CR and EOR, respectively. All figures are dorsal views of the ovary. Yellow asterisk indicates opening of the infundibulum for reference. Scale bar – 100 um.

    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) Crl:CD1(ICR) Charles River Strain code: 022; RRID:IMSR_ CRL:022 Strain, strain background (M. musculus) C57BL/6J Jackson Laboratory Stock #:000664; RRID:IMSR_ JAX:000664 Genetic reagent (M. musculus) Tre- H2B- Gfp (Tg(tetO- HIST1H2BJ/ GFP)47Efu/J) PMID:14671312 MGI:J:90563; RRID:IMSR_ JAX:005104 Genetic reagent (M. musculus) Tre- Cre (B6.Cg- Tg(tetO- cre)1Jaw/J) PMID:12145322 MGI:J:78365; RRID:IMSR_ JAX:006234 Genetic reagent (M. musculus) mTmG Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J PMID:17868096 MGI:J:124702; RRID:IMSR_ JAX:007576 Genetic reagent (M. musculus) Pax8- rtTA (B6.Cg- Tg(Pax8rtTA2S*M2)1Koes/J) PMID:18724376 MGI:J:140925; RRID:IMSR_ JAX:007176 Genetic reagent (M. musculus) Lgr5 (B6.129P2- Lgr5tm1(cre/ERT2)Cle/J) PMID:17934449 MGI:J:127123; RRID:IMSR_ JAX:008875 Antibody Smooth muscle alpha action (aSMA) (Cy3conjugated mouse monoclonal) Sigma- Aldrich C6198; RRID:AB_476856 (1:1000) Antibody E- Cadherin (rat monoclonal) Zymed (Thermo Fisher Scientific) 13- 1900; RRID:AB_2533005 (1:500) Antibody Endomucin (rat monoclonal) Santa Cruz Biotechnology sc- 65495; RRID:AB_2100037 (1:500) Antibody GFP (chicken polyclonal) Abcam ab13970; RRID:AB_300798 (1:1000) Antibody GFRa1 (goat polyclonal) R&D Systems AF560; RRID:AB_2110307 (1:150) Antibody KRT8 (rat monoclonal) DSHB TROMA- I; RRID:AB_531826 (1:250) Antibody PAX8 (rabbit polyclonal) Proteintech A10336- 1- AP; RRID:AB_2918972 (1:500) Antibody CNN1 (rabbit polyclonal) Proteintech 13938- 1- AP; RRID:AB_2082010 (1:200) Antibody ARL13b (rabbit polyclonal) Proteintech 17711- 1- AP; RRID:AB_2060867 (1:1000) Antibody RAB11 (rabbit monoclonal) Cell Signaling Technology 5589; RRID:AB_10693925 (1:500) Anbarci et al. eLife 2024;13:RP96662.

    Techniques:

    Figure 2. The extraovarian rete (EOR) is highly integrated with its extraovarian environment suggesting multifaceted communication. (a) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for ENDOMUCIN (magenta) and PAX8 (cyan). Bottom panel is an optical section showing vasculature tightly surrounding the distal dilated tip (DDT) of the EOR (magenta). (b) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for aSMA (magenta) and KRT8 (cyan). Bottom panel is an optical section showing the EOR tightly ensheathed by smooth muscle (magenta). (c) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for TUJ1 (magenta) and PAX8 (cyan). Bottom panel is an optical section showing direct contacts between the EOR and neuronal projections (magenta). (d) EOR at P0 immunostained for PAX8 (yellow), F4/80 (magenta), and LYVE-1 (cyan). Top and bottom panels are maximum intensity projection of the confocal Z-stack. Bottom panel shows the absence of LYVE1 macrophages (cyan) proximate to the EOR, in contrast to the closely associated F4/80 macrophages (magenta in top image). Arrowheads show regions devoid of LYVE1 macrophages where F4/80 macrophages are present. Scale bar – 100 um.

    Journal: eLife

    Article Title: Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary

    doi: 10.7554/elife.96662

    Figure Lengend Snippet: Figure 2. The extraovarian rete (EOR) is highly integrated with its extraovarian environment suggesting multifaceted communication. (a) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for ENDOMUCIN (magenta) and PAX8 (cyan). Bottom panel is an optical section showing vasculature tightly surrounding the distal dilated tip (DDT) of the EOR (magenta). (b) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for aSMA (magenta) and KRT8 (cyan). Bottom panel is an optical section showing the EOR tightly ensheathed by smooth muscle (magenta). (c) Top panel is a whole ovarian complex maximum intensity projection of the confocal Z-stack at E18.5 immunostained for TUJ1 (magenta) and PAX8 (cyan). Bottom panel is an optical section showing direct contacts between the EOR and neuronal projections (magenta). (d) EOR at P0 immunostained for PAX8 (yellow), F4/80 (magenta), and LYVE-1 (cyan). Top and bottom panels are maximum intensity projection of the confocal Z-stack. Bottom panel shows the absence of LYVE1 macrophages (cyan) proximate to the EOR, in contrast to the closely associated F4/80 macrophages (magenta in top image). Arrowheads show regions devoid of LYVE1 macrophages where F4/80 macrophages are present. Scale bar – 100 um.

    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) Crl:CD1(ICR) Charles River Strain code: 022; RRID:IMSR_ CRL:022 Strain, strain background (M. musculus) C57BL/6J Jackson Laboratory Stock #:000664; RRID:IMSR_ JAX:000664 Genetic reagent (M. musculus) Tre- H2B- Gfp (Tg(tetO- HIST1H2BJ/ GFP)47Efu/J) PMID:14671312 MGI:J:90563; RRID:IMSR_ JAX:005104 Genetic reagent (M. musculus) Tre- Cre (B6.Cg- Tg(tetO- cre)1Jaw/J) PMID:12145322 MGI:J:78365; RRID:IMSR_ JAX:006234 Genetic reagent (M. musculus) mTmG Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J PMID:17868096 MGI:J:124702; RRID:IMSR_ JAX:007576 Genetic reagent (M. musculus) Pax8- rtTA (B6.Cg- Tg(Pax8rtTA2S*M2)1Koes/J) PMID:18724376 MGI:J:140925; RRID:IMSR_ JAX:007176 Genetic reagent (M. musculus) Lgr5 (B6.129P2- Lgr5tm1(cre/ERT2)Cle/J) PMID:17934449 MGI:J:127123; RRID:IMSR_ JAX:008875 Antibody Smooth muscle alpha action (aSMA) (Cy3conjugated mouse monoclonal) Sigma- Aldrich C6198; RRID:AB_476856 (1:1000) Antibody E- Cadherin (rat monoclonal) Zymed (Thermo Fisher Scientific) 13- 1900; RRID:AB_2533005 (1:500) Antibody Endomucin (rat monoclonal) Santa Cruz Biotechnology sc- 65495; RRID:AB_2100037 (1:500) Antibody GFP (chicken polyclonal) Abcam ab13970; RRID:AB_300798 (1:1000) Antibody GFRa1 (goat polyclonal) R&D Systems AF560; RRID:AB_2110307 (1:150) Antibody KRT8 (rat monoclonal) DSHB TROMA- I; RRID:AB_531826 (1:250) Antibody PAX8 (rabbit polyclonal) Proteintech A10336- 1- AP; RRID:AB_2918972 (1:500) Antibody CNN1 (rabbit polyclonal) Proteintech 13938- 1- AP; RRID:AB_2082010 (1:200) Antibody ARL13b (rabbit polyclonal) Proteintech 17711- 1- AP; RRID:AB_2060867 (1:1000) Antibody RAB11 (rabbit monoclonal) Cell Signaling Technology 5589; RRID:AB_10693925 (1:500) Anbarci et al. eLife 2024;13:RP96662.

    Techniques:

    Figure 4. The extraovarian rete (EOR) and connecting rete (CR) acquire the potential for fluid transfer. (a–d) Cells of the CR acquire E-CADHERIN soon after birth. Maximum intensity projection from confocal Z-stacks of whole ovary/mesonephros complexes at E16.5-P7 immunostained for PAX8 (magenta) and E-CADHERIN (cyan). Arrowhead shows the region of the CR where E-CADHERIN expression is negligible at E16.5 (a), low at E18.5 (b), and fully expressed at P0 (c) and P7 (d). (e) Optical section from confocal Z-stacks of RO complexes at P0 immunostained for E-CADHERIN (yellow), CNN1 (magenta), and aSMA (cyan). Boxed regions show the absence of CNN1 in the aSMA+ sheath around the EOR at P0, and (f) its acquisition by P7, suggesting gain of contractility. (g) The RO expresses cilia marker ARL13b by P0. Maximum intensity projection from confocal Z-stacks of RO complexes at E18.5 immunostained for E-CADHERIN (cyan) and ARL13b (magenta). Outlined boxes show all regions of the EOR have ciliated cells. Scale bar – 100 um. (h) Airyscan optical section from confocal Z-stacks of RO complexes at E18.5 immunostained for KRT8 (cyan) and ARL13b (magenta). Scale bar – 25 um.

    Journal: eLife

    Article Title: Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary

    doi: 10.7554/elife.96662

    Figure Lengend Snippet: Figure 4. The extraovarian rete (EOR) and connecting rete (CR) acquire the potential for fluid transfer. (a–d) Cells of the CR acquire E-CADHERIN soon after birth. Maximum intensity projection from confocal Z-stacks of whole ovary/mesonephros complexes at E16.5-P7 immunostained for PAX8 (magenta) and E-CADHERIN (cyan). Arrowhead shows the region of the CR where E-CADHERIN expression is negligible at E16.5 (a), low at E18.5 (b), and fully expressed at P0 (c) and P7 (d). (e) Optical section from confocal Z-stacks of RO complexes at P0 immunostained for E-CADHERIN (yellow), CNN1 (magenta), and aSMA (cyan). Boxed regions show the absence of CNN1 in the aSMA+ sheath around the EOR at P0, and (f) its acquisition by P7, suggesting gain of contractility. (g) The RO expresses cilia marker ARL13b by P0. Maximum intensity projection from confocal Z-stacks of RO complexes at E18.5 immunostained for E-CADHERIN (cyan) and ARL13b (magenta). Outlined boxes show all regions of the EOR have ciliated cells. Scale bar – 100 um. (h) Airyscan optical section from confocal Z-stacks of RO complexes at E18.5 immunostained for KRT8 (cyan) and ARL13b (magenta). Scale bar – 25 um.

    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) Crl:CD1(ICR) Charles River Strain code: 022; RRID:IMSR_ CRL:022 Strain, strain background (M. musculus) C57BL/6J Jackson Laboratory Stock #:000664; RRID:IMSR_ JAX:000664 Genetic reagent (M. musculus) Tre- H2B- Gfp (Tg(tetO- HIST1H2BJ/ GFP)47Efu/J) PMID:14671312 MGI:J:90563; RRID:IMSR_ JAX:005104 Genetic reagent (M. musculus) Tre- Cre (B6.Cg- Tg(tetO- cre)1Jaw/J) PMID:12145322 MGI:J:78365; RRID:IMSR_ JAX:006234 Genetic reagent (M. musculus) mTmG Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J PMID:17868096 MGI:J:124702; RRID:IMSR_ JAX:007576 Genetic reagent (M. musculus) Pax8- rtTA (B6.Cg- Tg(Pax8rtTA2S*M2)1Koes/J) PMID:18724376 MGI:J:140925; RRID:IMSR_ JAX:007176 Genetic reagent (M. musculus) Lgr5 (B6.129P2- Lgr5tm1(cre/ERT2)Cle/J) PMID:17934449 MGI:J:127123; RRID:IMSR_ JAX:008875 Antibody Smooth muscle alpha action (aSMA) (Cy3conjugated mouse monoclonal) Sigma- Aldrich C6198; RRID:AB_476856 (1:1000) Antibody E- Cadherin (rat monoclonal) Zymed (Thermo Fisher Scientific) 13- 1900; RRID:AB_2533005 (1:500) Antibody Endomucin (rat monoclonal) Santa Cruz Biotechnology sc- 65495; RRID:AB_2100037 (1:500) Antibody GFP (chicken polyclonal) Abcam ab13970; RRID:AB_300798 (1:1000) Antibody GFRa1 (goat polyclonal) R&D Systems AF560; RRID:AB_2110307 (1:150) Antibody KRT8 (rat monoclonal) DSHB TROMA- I; RRID:AB_531826 (1:250) Antibody PAX8 (rabbit polyclonal) Proteintech A10336- 1- AP; RRID:AB_2918972 (1:500) Antibody CNN1 (rabbit polyclonal) Proteintech 13938- 1- AP; RRID:AB_2082010 (1:200) Antibody ARL13b (rabbit polyclonal) Proteintech 17711- 1- AP; RRID:AB_2060867 (1:1000) Antibody RAB11 (rabbit monoclonal) Cell Signaling Technology 5589; RRID:AB_10693925 (1:500) Anbarci et al. eLife 2024;13:RP96662.

    Techniques: Expressing, Marker

    Figure 5. Presence of SNARE-complex members suggests a role for secretion. (a) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) extraovarian rete (EOR) at E18.5 using hybridization chain reaction (HCR) for the detection of Clu expression (magenta). (b) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at E18.5 using HCR for Stxbp2 (magenta). (c) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for Clu (magenta). (d) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for Stxbp2 (magenta). (e) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-Cre; Rosa26mTmG (cyan) EOR at E18.5 immunostained for STX3 (magenta). Outlined higher resolution image acquired with Airyscan. (f) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-Cre; Rosa26mTmG (cyan) EOR at E18.5 immunostained for RAB11 (magenta). Outlined higher resolution image acquired with Airyscan. Scale bar – 100 um.

    Journal: eLife

    Article Title: Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary

    doi: 10.7554/elife.96662

    Figure Lengend Snippet: Figure 5. Presence of SNARE-complex members suggests a role for secretion. (a) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) extraovarian rete (EOR) at E18.5 using hybridization chain reaction (HCR) for the detection of Clu expression (magenta). (b) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at E18.5 using HCR for Stxbp2 (magenta). (c) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for Clu (magenta). (d) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for Stxbp2 (magenta). (e) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-Cre; Rosa26mTmG (cyan) EOR at E18.5 immunostained for STX3 (magenta). Outlined higher resolution image acquired with Airyscan. (f) Optical section from confocal Z-stacks of PAX8-rtTa; Tre-Cre; Rosa26mTmG (cyan) EOR at E18.5 immunostained for RAB11 (magenta). Outlined higher resolution image acquired with Airyscan. Scale bar – 100 um.

    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) Crl:CD1(ICR) Charles River Strain code: 022; RRID:IMSR_ CRL:022 Strain, strain background (M. musculus) C57BL/6J Jackson Laboratory Stock #:000664; RRID:IMSR_ JAX:000664 Genetic reagent (M. musculus) Tre- H2B- Gfp (Tg(tetO- HIST1H2BJ/ GFP)47Efu/J) PMID:14671312 MGI:J:90563; RRID:IMSR_ JAX:005104 Genetic reagent (M. musculus) Tre- Cre (B6.Cg- Tg(tetO- cre)1Jaw/J) PMID:12145322 MGI:J:78365; RRID:IMSR_ JAX:006234 Genetic reagent (M. musculus) mTmG Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J PMID:17868096 MGI:J:124702; RRID:IMSR_ JAX:007576 Genetic reagent (M. musculus) Pax8- rtTA (B6.Cg- Tg(Pax8rtTA2S*M2)1Koes/J) PMID:18724376 MGI:J:140925; RRID:IMSR_ JAX:007176 Genetic reagent (M. musculus) Lgr5 (B6.129P2- Lgr5tm1(cre/ERT2)Cle/J) PMID:17934449 MGI:J:127123; RRID:IMSR_ JAX:008875 Antibody Smooth muscle alpha action (aSMA) (Cy3conjugated mouse monoclonal) Sigma- Aldrich C6198; RRID:AB_476856 (1:1000) Antibody E- Cadherin (rat monoclonal) Zymed (Thermo Fisher Scientific) 13- 1900; RRID:AB_2533005 (1:500) Antibody Endomucin (rat monoclonal) Santa Cruz Biotechnology sc- 65495; RRID:AB_2100037 (1:500) Antibody GFP (chicken polyclonal) Abcam ab13970; RRID:AB_300798 (1:1000) Antibody GFRa1 (goat polyclonal) R&D Systems AF560; RRID:AB_2110307 (1:150) Antibody KRT8 (rat monoclonal) DSHB TROMA- I; RRID:AB_531826 (1:250) Antibody PAX8 (rabbit polyclonal) Proteintech A10336- 1- AP; RRID:AB_2918972 (1:500) Antibody CNN1 (rabbit polyclonal) Proteintech 13938- 1- AP; RRID:AB_2082010 (1:200) Antibody ARL13b (rabbit polyclonal) Proteintech 17711- 1- AP; RRID:AB_2060867 (1:1000) Antibody RAB11 (rabbit monoclonal) Cell Signaling Technology 5589; RRID:AB_10693925 (1:500) Anbarci et al. eLife 2024;13:RP96662.

    Techniques: Hybridization, Expressing

    Figure 6. Validation of Igfbp2 expression in the rete ovarii (RO). (a) Maximum intensity projection of confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) extraovarian rete (EOR) at E18.5 using hybridization chain reaction (HCR) for the detection of IGFBP2 expression (magenta). (b) Maximum intensity projection of confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for the detection of IGFBP2 expression (magenta). Bottom panels are outlined. Higher resolution images acquired with Airyscan. Scale bar top panel – 100 um. Scale bar bottom panel – 25 um.

    Journal: eLife

    Article Title: Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary

    doi: 10.7554/elife.96662

    Figure Lengend Snippet: Figure 6. Validation of Igfbp2 expression in the rete ovarii (RO). (a) Maximum intensity projection of confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) extraovarian rete (EOR) at E18.5 using hybridization chain reaction (HCR) for the detection of IGFBP2 expression (magenta). (b) Maximum intensity projection of confocal Z-stacks of PAX8-rtTa; Tre-H2B-GFP (cyan) EOR at P7/8 using HCR for the detection of IGFBP2 expression (magenta). Bottom panels are outlined. Higher resolution images acquired with Airyscan. Scale bar top panel – 100 um. Scale bar bottom panel – 25 um.

    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Mus musculus) Crl:CD1(ICR) Charles River Strain code: 022; RRID:IMSR_ CRL:022 Strain, strain background (M. musculus) C57BL/6J Jackson Laboratory Stock #:000664; RRID:IMSR_ JAX:000664 Genetic reagent (M. musculus) Tre- H2B- Gfp (Tg(tetO- HIST1H2BJ/ GFP)47Efu/J) PMID:14671312 MGI:J:90563; RRID:IMSR_ JAX:005104 Genetic reagent (M. musculus) Tre- Cre (B6.Cg- Tg(tetO- cre)1Jaw/J) PMID:12145322 MGI:J:78365; RRID:IMSR_ JAX:006234 Genetic reagent (M. musculus) mTmG Gt(ROSA)26Sortm4(ACTBtdTomato,-EGFP)Luo/J PMID:17868096 MGI:J:124702; RRID:IMSR_ JAX:007576 Genetic reagent (M. musculus) Pax8- rtTA (B6.Cg- Tg(Pax8rtTA2S*M2)1Koes/J) PMID:18724376 MGI:J:140925; RRID:IMSR_ JAX:007176 Genetic reagent (M. musculus) Lgr5 (B6.129P2- Lgr5tm1(cre/ERT2)Cle/J) PMID:17934449 MGI:J:127123; RRID:IMSR_ JAX:008875 Antibody Smooth muscle alpha action (aSMA) (Cy3conjugated mouse monoclonal) Sigma- Aldrich C6198; RRID:AB_476856 (1:1000) Antibody E- Cadherin (rat monoclonal) Zymed (Thermo Fisher Scientific) 13- 1900; RRID:AB_2533005 (1:500) Antibody Endomucin (rat monoclonal) Santa Cruz Biotechnology sc- 65495; RRID:AB_2100037 (1:500) Antibody GFP (chicken polyclonal) Abcam ab13970; RRID:AB_300798 (1:1000) Antibody GFRa1 (goat polyclonal) R&D Systems AF560; RRID:AB_2110307 (1:150) Antibody KRT8 (rat monoclonal) DSHB TROMA- I; RRID:AB_531826 (1:250) Antibody PAX8 (rabbit polyclonal) Proteintech A10336- 1- AP; RRID:AB_2918972 (1:500) Antibody CNN1 (rabbit polyclonal) Proteintech 13938- 1- AP; RRID:AB_2082010 (1:200) Antibody ARL13b (rabbit polyclonal) Proteintech 17711- 1- AP; RRID:AB_2060867 (1:1000) Antibody RAB11 (rabbit monoclonal) Cell Signaling Technology 5589; RRID:AB_10693925 (1:500) Anbarci et al. eLife 2024;13:RP96662.

    Techniques: Biomarker Discovery, Expressing, Hybridization